Hatching and freezing gemmules from the freshwater sponge Ephydatia muelleri
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The protocol includes: fixing sponges, blocking tissue from non-specific binding, applying primary and secondary antibody label, counterstaining, mounting on slides, and making MOWIOL medium.
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Quantifying Sneezing in sponges:
ImageJ script for measuring changes in area of a sponge to quantify sneezing |